ABSTRACT
A type of entomopathogenic fungus of soil in Citrus grandis 'tomentosa' production base was isolated and identified with morphological and molecular biological methods, including pathogenesis, spore characteristic and ITS sequence analysis were conducted. The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples, which were identified as Metarhizium anisopliae var. anisopliae. Based on results above, we concluded that there was quantity of Metarhizium resources in this area. These provided the useful information for controlling some pests of C. grandis by using these strains of fungus.
Subject(s)
Animals , Citrus , Parasitology , Metarhizium , Physiology , Pest Control, Biological , Methods , Soil MicrobiologyABSTRACT
In order to explore the environmental pest management method of Dorysthenes hydropicus, three strains of entomopathogenic nematodes, viz. Heterorhabditis bacteriphora (H06), Steinernema scapterisci (SS), S. carpocapsae (All) were used on larvae of Dorysthenes hydropicus, with treatments of 0, 5 000 and 10 000 nematodes each larva. The result showed that these three strains viz. All, H06 and SS had high lethal effects on the larvae. Lethal rates had dose-effects relationship with inoculation amounts. High dose treatments resulted in high mortalities and led to quick death, especially in the treatment of H06. Treatment of H06 with 10 000 nematodes per larva resulted in 100% mortality after inoculated 4 days. Different strains of these nematodes had various lethal characters, H06 with only one peak mortality, the larvae died quickly after inoculated, while All and SS with 2 peak mortalities, there was a stable stage with low mortality between the 2 peak mortalities. Entomopathogenic nematodes could be used as a hopeful method for controlling of Dorysthenes hydropicus in fields.